丁香实验_LOGO
登录
提问
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

A Transplant Model for Human Epidermal Skin Regeneration

互联网

363
This protocol describes a technically simple in vivo assay of long-term skin regeneration in human skin, providing a reliable method for epidermal tissue reconstitution using small numbers of several types of epithelial cells, from epithelial cell lines to primary epithelial stem cells and transplanted with or without prior culture. The model relies on the repopulation of devitalized rat tracheas by human keratinocyte suspensions following subcutaneous transplantation into immunodeficient mice. Here, we describe complete optimization and characterization of this model for robust regeneration of epithelium from cell suspensions of a limited number of primary human keratinocytes.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序