Measuring Transcription of Metalloproteinase Genes: Nuclear Run-Off Assay vs Analysis of hnRNA

互联网2014-02-13

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Many metalloproteinase genes are transcriptionally regulated by differentiation, growth factors, cytokines, and hormones. Nuclear run-off assays and analysis of heterogeneous nuclear RNA (hnRNA) levels are two methods for studying changes in gene transcription. In this chapter these two techniques will be outlined and compared.

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