丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Preparation of Horseradish Peroxidase-Labeled Probes

互联网

767
The direct labeling of nucleic acid probes with horseradish peroxidase (HRP) was first described by Renz at EMBL in 1984 (1 ). The methodology was combined with enhanced chemiluminescence (2 ) (a light producing HRP catalyzed reaction; see chapter 20 ) allowing the detection of specific hybrids on membranes (3 ). Further development led to the availability of the first light-based nucleic acid detection system; this was capable of detecting 2.5 pg of target nucleic acid on genomic Southern blots (4 ). Subsequent protocol and reagent improvements now enable researchers to reliably detect 0.5 pg of target nucleic acid.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序