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Antibody Purificatioin
- Purification of IgG Using Protein A- or Protein G-Agarose
(KPL)
- Purifying Antibodies (Perkin-Elmer)
Precipitation Methods
Protein A or Protein G Columns or Beads
Antigen Columns
・ Affinity Purification of Antibodies from Crude Serum (PMCI)
・ Affinity Purification of Antibody (Walter Steffen)
- Coupling procedure
- Affinity purification
- Absorption of antibodies to acetone powder
- Production of acetone powder
- Extraction of antibodies from egg yolk
- Purification and cleavage of murine monoclonal antibodies
- IgG purification with protein G
- Digest of IgG with papain
・ Affinity Purification of Antibodies (Vesicle Trafficking)
・ Affinity Purification of Antibodies
(XMMR)
protocol in great detail, including column preparation, wash and more...
・ Protein G Purification of Antibodies (Contributed by Nanci Donacki)
・ Protein A Purification of Antibody (Contributed by Nanci Donacki)
- Coupling Antibodies to Protein A/G
(Breeden Lab)
- Coupling Peptides to Resin for Affinity Purification
(Mitshison Lab)
- Purification of Anti Peptide Antibody
(Mitshison Lab)
- Antibody Purification
(Mike A. Dyer)
This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.
Column Preparation
- Making Affinity Columns (XMMR)
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