Cerebellar Slice Cultures

Cerebellar slice cultures are a versatile method to study cerebellar cells in an in vitro preparation that preserves many aspects of the microenvironment of the cerebellar tissue. The setup of this slice culture model is easy, possible throughout the first two postnatal weeks in rodents, and does not require sophisticated equipment. Cerebellar slice cultures can be used for the study of electrophysiological properties of cerebellar neurons, for studying cerebellar development and various aspects of the biology of cerebellar cells. Several antibodies are available, which can be used as cell-specific markers for most cerebellar neurons and allow a good visualization of their morphology. Cerebellar slice cultures reduce the need for in vivo experiments and have developed into an important tool for the study of cerebellar biology.