Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprinting Spectra
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This chapter describes a number of aspects important for calibration of matrix-assisted laser desorption/ionization time-of-flight
spectra prior to peptide mass fingerprinting searches. Both multipoint internal calibration and mass defect-based calibration
is illustrated. The chapter describes how potential internal calibrants, like tryptic autodigest peptides and keratin-related
peptides, can be identified and used for high-precision calibration. Furthermore, the construction of project/user-specific
lists of potential calibrants is illustrated.
