Production of Recombinant Bispecific Antibodies

Recombinant bispecific antibodies have great potential as diagnostic and therapeutic reagents (1 ,2 ). Applications of recombinant bispecific antibodies include the recruitment of effector molecules (e.g., enzymes, complement components, immunoglobulins), effector cells (cytotoxic T-lymphocytes, natural killer [NK] cells, macrophages), or gene-therapeutic vectors (e.g., adenoviruses) to other molecules or target cells (3 –10 ). Through genetic engineering, molecules with the desired properties can be generated and produced in various prokaryotic or eukaryotic expression systems (11 ). For example, by starting from antibody fragments isolated from human combinatorial libraries by phage display, completely human bispecific molecules can be generated.