Autoradiographic Localization and Quantification of Components of the Renin-Angiotensin System in Tissues
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In situ radioligand binding with autoradiography allows localization and quantification of bound radiolabeled ligands in tissues. This is a very sensitive technique that enables the characterization of binding kinetics and ligand specificity and the quantification of the amount of radioligand bound in different structures within the tissue. This technique is complementary to the higher resolution of immunohistochemical localization of proteins or binding sites on fixed tissue sections and in situ hybridization histochemical localization of mRNA.
