Folate-Polylysine-Mediated Delivery of a Multiunit Anti-BCR/ABL Ribozyme to BCR/ABL-Transformed 32D Cells

The use of oligodeoxynucleotides (ODN) to disrupt gene function has been studied in a variety of in vitro and in vivo systems (1 –14 ). Antigene, antisense, ribozyme, and aptamer nucleic acid molecules have been shown in numerous model systems to effect DNA, RNA, or protein targets, or physiologic properties of cells (15 ). However, nucleic acid drugs must overcome several problems before wide application of these promising strategies can be realized.