Cloning by Limiting Dilution of Hybridoma

Cloning by Limiting Dilution of Hybridoma

1.   DMEM, high glucose (Life Technologies, Inc. #10313-021 or equivalent)
2.   Fetal Bovine Serum (Life Technologies, Inc. #16000-044 or equivalent)
3.   L-glutamine (Life Technologies, Inc. #25030-149 or equivalent)
4.   Hybridoma Cloning Factor (Fisher # IG50-0615)
5. 50 ml sterile centrifuge tubes ( Falcon #2070)
6. 15 ml sterile centrifuge tubes (Falcon # 2099)
7. 96 well culture plates (Falcon #3072))
8.   Hemocytometer
9. Trypan Blue, 0.4% (Life Technologies, Inc. # 630-5150AG)
10. Multi-channel pipettor and sterile tips
11. Reagent Reservoir
12. HT (Life Technologies, Inc. #11067-030)