Long-Range PCR
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The heat-stable DNA polymerase utilized in the polymerase chain reaction (PCR) has been widely used to amplify DNA fragments since its conception by Kerry Mullis in 1985. However, it soon became apparent that there was a constraint on the maximum size of amplified fragments. For genomic DNA, this was 3–4 kb (1 ), whereas for phage lambda DNA amplifications of up to 15 kb have been possible (2 ).
