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Incorporation of Modified Bases into Oligonucleotides

互联网

354
Nucleobases with attached side chains have been incorporated into oligodeoxynucleotides (ODNs) to achieve a number of goals. The functionalized side chains have been used to attach reporter groups (protein ligands as well as fluorescent moieties) and metal-chelating groups. Electrophilic and photochemical alkylating functions have been attached to react with the other strand(s) in a duplex (or triplex).
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