【共享】“KOZAK CONSENSUS SEQUENCE”简介
丁香园论坛
1949
本是想回答一个网友的相关问题，可我找不到他/她的贴在哪儿，只好自己发一贴。
谈一下：有关“KOZAK CONSENSUS SEQUENCE”
I.Kozak Sequence
http://www.sciencegateway.org/resources/kozak.htm
Most eukaryotic mRNAs contain a short recognition sequence that greatly facilitate the initial binding of mRNA to the small subunit of the ribosome. The consensus sequence for initiation of translation in vertebrates (also called Kozak sequence) is:
ACCATGG
More general it is:
(GCC)RCCATGG
where R is a purine (A or G).
To improve expression levels, it may be advantageous to design the cloned insert according to Kozak's rules.
References:
Kozak, M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986, 44(2):283-92.
Kozak, M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987, 15(20):8125-48
II.我再补充一些材料：
1。真核mRNA起始密码旁侧的共有序列：
分类 共有序列 文献
脊椎动物 GCCGCCA/GCC A （+1 ） UGG Kozzak M ,Nucleic Acids ,
R es,1 9 87,15:8125
植物 AACAAA A （+1 ） UGGC Kozzak M ,J.Biol Chem,1991,
226:8 125
Kozzak M ,J.Cell.Biol,1991,115:887
原生动物 A/U A/U A/U A/U A/U A/U --A -- Yamauchi K ,Nucleic Acids
A/U A/U A/U--AAA A （+1） UGAN
Res,1991,19:2715,
Yeast A/U-A-A/U-A A（ +1） UGUCU Cigam A m et al Genes,
1987,
59:9:1
2。EXTRACTING KOZAK CONSENSUS SEQUENCE USING KLEISLI.
http://www.bionet.nsc.ru/bgrs/thesis/61/
排版真难呀！
3。几个附件：
http://www.bioteq.org/bbs/viewthread.php?tid=2900&pid=10251&page=1#pid10251
在构建表达载体时这个KOZAK CONSENSUS SEQUENCE因素还是好好考虑的。
谈一下：有关“KOZAK CONSENSUS SEQUENCE”
I.Kozak Sequence
http://www.sciencegateway.org/resources/kozak.htm
Most eukaryotic mRNAs contain a short recognition sequence that greatly facilitate the initial binding of mRNA to the small subunit of the ribosome. The consensus sequence for initiation of translation in vertebrates (also called Kozak sequence) is:
ACCATGG
More general it is:
(GCC)RCCATGG
where R is a purine (A or G).
To improve expression levels, it may be advantageous to design the cloned insert according to Kozak's rules.
References:
Kozak, M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986, 44(2):283-92.
Kozak, M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987, 15(20):8125-48
II.我再补充一些材料：
1。真核mRNA起始密码旁侧的共有序列：
分类 共有序列 文献
脊椎动物 GCCGCCA/GCC A （+1 ） UGG Kozzak M ,Nucleic Acids ,
R es,1 9 87,15:8125
植物 AACAAA A （+1 ） UGGC Kozzak M ,J.Biol Chem,1991,
226:8 125
Kozzak M ,J.Cell.Biol,1991,115:887
原生动物 A/U A/U A/U A/U A/U A/U --A -- Yamauchi K ,Nucleic Acids
A/U A/U A/U--AAA A （+1） UGAN
Res,1991,19:2715,
Yeast A/U-A-A/U-A A（ +1） UGUCU Cigam A m et al Genes,
1987,
59:9:1
2。EXTRACTING KOZAK CONSENSUS SEQUENCE USING KLEISLI.
http://www.bionet.nsc.ru/bgrs/thesis/61/
排版真难呀！
3。几个附件：
http://www.bioteq.org/bbs/viewthread.php?tid=2900&pid=10251&page=1#pid10251
在构建表达载体时这个KOZAK CONSENSUS SEQUENCE因素还是好好考虑的。
