Using the SELEX Combinatorial Chemistry Process to Find High Affinity Nucleic Acid Ligands to Target Molecules
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The synthesis of a template containing a variable region flanked by 5′ and 3′ fixed regions that enable amplification by PCR,
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Conversion of the template to an amplified library of candidate ligands,
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A binding reaction in which a fraction of the candidate ligands are bound by the target molecule;
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Separation of target molecule/ligand complexes from unbound candidate ligands;
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Purification of the nucleic acid ligands from the separated complexes; and
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Replication of the nucleic acid ligand population.
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