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Purification of MMPs and TIMPs

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Currently more than twenty different matrix metalloproteinases (MMPs) and four tissue inhibitors of metalloproteinases (TIMPs) have been identified (1 ,2 ) by cDNA cloning. This chapter describes methods to purify collagenases (MMP-1, MMP-8, and MMP-13), gelatinases (MMP-2 and MMP-9), stromelysins (MMP-3 and MMP-10), macrophage elastase (MMP-12), and TIMP-1 and TIMP-2 from natural sources.
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