In Vitro DNA Recombination by Random Priming

Variation coupled to selection is the hallmark of natural evolution. Although there is no full agreement concerning the best way to create variation, mutation or recombination (1 ), computational simulation studies have demonstrated the importance of homologous recombination in the evolution of biological systems (2 ,3 ). As compared to random mutagenesis, recombination may be advantageous in combining beneficial mutations that have arisen independently and may be synergistic, while simultaneously removing deleterious mutations.