Lactobacillus culture
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Overview
General overview and guidelines on how to grow up a culture of Lactobacillus
Materials
Procedure
- Follow instructions to make MRS plates and media, autoclave to sterilize
- Streak Lactobacillus on MRS plate or inoculate into test tube of MRS media
- Incubate at 37C under anaerobic conditions
- If from new source of Lactobacillus (ex. dehydrated bacteria from ATCC) allow up to 3 days to grow. Otherwise, allow 12-24 hours to grow
