How long can bisulfite-treated DNA be stored-DNA Methyla

Quick question as I have read all sorts of views on this before:

How long can bisufite-treated DNA be stored in -80℃ freezer before it degrades. Is there any reason why it should degrade quicker than untreated DNA??

Jon

-spjgjsm-

--------------------------------------------------------------------------------

Jon,

I resuspend my bisulfite treated DNA in fresh autoclaved TE buffer and store at -20C. I have stored such DNA upto 6 months with no detectable loss of DNA and by then I have used up my batch and I have to make another batch.

Hope this helps.

Cheers

Nick

-methylnick-

--------------------------------------------------------------------------------

I agree with Nick. No problem for six months although some suggest no more than one month.

-pcrman-

--------------------------------------------------------------------------------

cheers!

jon

-spjgjsm-

--------------------------------------------------------------------------------

Make sure you store in TE, rather than water. Bis treated DNA stored in water goes off quite fast.

-Chris Harris-

--------------------------------------------------------------------------------