【求助】PPARr拮抗剂GW9662的作用?
丁香园论坛
2208
我在文献中查到,PPARr拮抗剂GW9662可以抑制药物的抗炎作用,前提是药物是通过激活PPARr来实现其抗炎作用的。即炎症细胞中的炎症因子(如:NO)升高,加入抗炎药物后,NO水平降低,当同时给予GW9662和该抗炎药物时,该药物的抗炎作用被抑制,NO水平再次升高。以上是文献的报道,但是我在实验时发现,加入了抗炎药物+GW9662后,NO的水平不但没有升高,反而降低了(与单独抗炎药物组相比),不知这是怎么回事?
I think there may be a number of possibilities you may want to take into consideration:
1, have you set up a positive control with a known drug that works in the same way?
2, is increase on NO level the right indicator at all the conditions?
2, if the positive control turns out the predict result, it's possible your drug may exert its anti-inflamation effect not through the reported PPAR pathway. and GW9662 can potentiate the other anti-inflamation pathway. So you may want to check other anti-inflamation pathways.
Good luck!
1, have you set up a positive control with a known drug that works in the same way?
2, is increase on NO level the right indicator at all the conditions?
2, if the positive control turns out the predict result, it's possible your drug may exert its anti-inflamation effect not through the reported PPAR pathway. and GW9662 can potentiate the other anti-inflamation pathway. So you may want to check other anti-inflamation pathways.
Good luck!
回答得不错!
magichunter战友,您好,我现在实验过程 中遇到了和您一样的问题。在RAW264.7细胞加入罗格列酮,抑制了TNF的产生,罗格列酮+GW9662反而进一步抑制了TNF产生(和罗格列酮单用相比),不知什么原因?
您现在有没有高见,指点一下,非常感谢!
您现在有没有高见,指点一下,非常感谢!
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