The Use of a Continuous Culture System to Study the Antimicrobial Susceptibility of Bacteria in Biofilm

The classical method for determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of an antibiotic is the tube test. Details of this test are well known and recognized methods are published (1 ). The recent development of the E-test (AB Biotest, Solna, Sweden), has been a significant development in the determination of MIC. This system is straightforward to perform and is suitable for larger scale work.