SNP Detection Using Trityl Mass Tags
互联网
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A new method suitable for single nucleotide polymorphism (SNP) detection using differential oligonucleotide probe extension has been developed. Sulfur-linked laser-cleavable trityl labels are implemented in this protocol. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption–ionization of the cleavable labels. The usefulness of this method for SNP genotyping is demonstrated.