丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Rapid Isolation of Genomic DNA from Human Oral Mucosa

互联网

674

 

  1. Take an oral smear
  2. Dissolve in 50 ml H2 O through vigorous shaking
  3. Pellet cells 5 min at 4000 rpm
  4. Re-suspended the pellet in 400 μl Lysis buffer (50 mM TRIS-Cl pH 8, 10 mM EDTA, 2% SDS)
  5. Incubate for 5 min at 65¡ãC
  6. Add 250 μl 4.5 M NaCl and mix
  7. Pellet cellular debris (13000 rpm for 4 min). Genomic DNA stays in the supernatant
  8. Precipitate DNA by adding 650 μl of isopropanol to the supernatant and centrifuge 5 min at 13000 rpm
  9. Re-suspend the DNA in an appropriate buffer (e.g. TrisCl, pH=8.0)

 

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序