Rich Agarose Plates:

50 mM

NaCl

0.75 %

BactoPeptone

5 ug/ml

cholesteroundefined

1 mM

CaCl2 *

1 mM

MgSO4 *

25 mM

K-PO4 (pH 6.0~undefined

1.5 %

agarose (not agar)

~undefined add after autoclaving

Worm Lysis Solution:

100 mM

Tris (pH 8.5)

100 mM

NaCl

50 mM

EDTA

1 %

SDS

1 %

beta-mercaptoethanol

100 ug/ml

proteinase K

Phenol / CHCl3

25:24:1

Phenol : CHCl3 : isoamyl alcohol

CHCl3

24:1

CHCl3 : isoamyl alcohol