DAPI Staining
互联网
1026
<center> <p> <font color="#00005a"><b>DAPI Staining</b> </font></p> </center>
To visualize DNA, incubate fixed samples with 100 ng/ml 4',6'-diamidino-2-phenylindole hydrochloride (DAPI) in PBS for 30 min. Rinse 3x with PBTw.
Materials
PBS: Sambrook et al. (Molecular Cloning ).
PBTw: PBS containing 0.1% Tween 20.
DAPI: 100 ug/ml stock solution in dH2 O. Store at -20�.
