【原创】NCBI中查询出的cds序列中出现了k,r,m,请问代表什么意思呢?
丁香园论坛
3896
请各位战友看下面这段注释,来自NCBI:
{
LOCUS AY602989 704 bp DNA linear PLN 19-DEC-2006
DEFINITION Trichoderma sp. zd 56 endochitinase 42 (ech42) gene, partial cds.
ACCESSION AY602989
VERSION AY602989.1 GI:52630747
KEYWORDS .
SOURCE Trichoderma sp. zd 56
ORGANISM Trichoderma sp. zd 56
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae;
mitosporic Hypocreaceae; Trichoderma.
REFERENCE 1 (bases 1 to 704)
AUTHORS Druzhinina,I.S., Komon-Zelazowska,M., Bissett,J. and Kubicek,C.P.
TITLE The major phylogenetic lineages of the mycoparasitic fungus
Trichoderma harzianum form a recombining, panmictic population
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 704)
AUTHORS Zafari,D.
TITLE Direct Submission
JOURNAL Submitted (17-APR-2004) Department of Plant Protection, Bu Ali Sina
University, Hamadan, Iran
FEATURES Location/Qualifiers
source 1..704
/organism="Trichoderma sp. zd 56"
/mol_type="genomic DNA"
/strain="zd 56"
/db_xref="taxon:289995"
gene <1..>704
/gene="ech42"
mRNA <1..>704
/gene="ech42"
/product="endochitinase 42"
CDS <1..>704
/gene="ech42"
/codon_start=2
/product="endochitinase 42"
/protein_id="AAU84850.1"
/db_xref="GI:52630748"
/translation="FPSAASTDANRKNFARTAIAFMKDXGFBGIDVDWEYPADSTQAS
NMILLLKEVXSQLDAYAAQYXPGYHFLLTIAAPAGKDNYSKLXLADLGQVLBYINLMA
YBYAGSFSPLTGHBANLFANPSNPNATPFNTDTAVKDYINGGVPANKIVLGMPIYGRS
FQNTAGIGQTYNGVGGGGGGSTGSWEAGIWDYKALPRSGATIKYDDVAKGYYSYNANT
KELISFDTPDMINTKV"
ORIGIN
1 cttcccttct gcagcaagca cggatgccaa ccgaaagaac tttgcacgaa ctgccattgc
61 attcatgaag gatkggggtt tcratggcat tgacgtcgac tgggagtacc ctgccgacag
121 cacccaggct tccaacatga ttcttctgct caaggaagtc cratctcagc tggatgctta
181 tgctgcccaa tacscccccg gctaccactt cctcctmacc attgctgccc cagctggcaa
241 ggataactac tccaagctgc scctggctga tcttggccaa gtcctcract atattaacct
301 catggcctac ractacgctg gatccttcag ccccctcact ggccacracg ccaacctgtt
361 tgccaacccg tccaacccca atgccacacc cttcaacacc gacactgctg tcaaggatta
421 tatcaatgga ggtgttcccg caaacaagat tgttctcggc atgcccatct acggacgatc
481 attccagaac accgctggta ttggccagac ttacaacgga gttggaggtg gtggtggtgg
541 ctcaactggc agctgggagg ccggtatctg ggattacaag gctcttccca ggtccggcgc
601 caccatcaag tacgatgatg tcgcaaaggg ttactacagc tacaacgcca acaccaagga
661 gctcatctct ttcgataccc ctgacatgat caacaccaag gttg
//
}
奇怪的是序列中出现了r,m,k,请问是什么意思呢?
在NCBI的链接如下:
[url][/url]http://www.ncbi.nlm.nih.gov/nuccore/52630747?from=1&to=704&report=gbwithparts
{
LOCUS AY602989 704 bp DNA linear PLN 19-DEC-2006
DEFINITION Trichoderma sp. zd 56 endochitinase 42 (ech42) gene, partial cds.
ACCESSION AY602989
VERSION AY602989.1 GI:52630747
KEYWORDS .
SOURCE Trichoderma sp. zd 56
ORGANISM Trichoderma sp. zd 56
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae;
mitosporic Hypocreaceae; Trichoderma.
REFERENCE 1 (bases 1 to 704)
AUTHORS Druzhinina,I.S., Komon-Zelazowska,M., Bissett,J. and Kubicek,C.P.
TITLE The major phylogenetic lineages of the mycoparasitic fungus
Trichoderma harzianum form a recombining, panmictic population
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 704)
AUTHORS Zafari,D.
TITLE Direct Submission
JOURNAL Submitted (17-APR-2004) Department of Plant Protection, Bu Ali Sina
University, Hamadan, Iran
FEATURES Location/Qualifiers
source 1..704
/organism="Trichoderma sp. zd 56"
/mol_type="genomic DNA"
/strain="zd 56"
/db_xref="taxon:289995"
gene <1..>704
/gene="ech42"
mRNA <1..>704
/gene="ech42"
/product="endochitinase 42"
CDS <1..>704
/gene="ech42"
/codon_start=2
/product="endochitinase 42"
/protein_id="AAU84850.1"
/db_xref="GI:52630748"
/translation="FPSAASTDANRKNFARTAIAFMKDXGFBGIDVDWEYPADSTQAS
NMILLLKEVXSQLDAYAAQYXPGYHFLLTIAAPAGKDNYSKLXLADLGQVLBYINLMA
YBYAGSFSPLTGHBANLFANPSNPNATPFNTDTAVKDYINGGVPANKIVLGMPIYGRS
FQNTAGIGQTYNGVGGGGGGSTGSWEAGIWDYKALPRSGATIKYDDVAKGYYSYNANT
KELISFDTPDMINTKV"
ORIGIN
1 cttcccttct gcagcaagca cggatgccaa ccgaaagaac tttgcacgaa ctgccattgc
61 attcatgaag gatkggggtt tcratggcat tgacgtcgac tgggagtacc ctgccgacag
121 cacccaggct tccaacatga ttcttctgct caaggaagtc cratctcagc tggatgctta
181 tgctgcccaa tacscccccg gctaccactt cctcctmacc attgctgccc cagctggcaa
241 ggataactac tccaagctgc scctggctga tcttggccaa gtcctcract atattaacct
301 catggcctac ractacgctg gatccttcag ccccctcact ggccacracg ccaacctgtt
361 tgccaacccg tccaacccca atgccacacc cttcaacacc gacactgctg tcaaggatta
421 tatcaatgga ggtgttcccg caaacaagat tgttctcggc atgcccatct acggacgatc
481 attccagaac accgctggta ttggccagac ttacaacgga gttggaggtg gtggtggtgg
541 ctcaactggc agctgggagg ccggtatctg ggattacaag gctcttccca ggtccggcgc
601 caccatcaag tacgatgatg tcgcaaaggg ttactacagc tacaacgcca acaccaagga
661 gctcatctct ttcgataccc ctgacatgat caacaccaag gttg
//
}
奇怪的是序列中出现了r,m,k,请问是什么意思呢?
在NCBI的链接如下:
[url][/url]http://www.ncbi.nlm.nih.gov/nuccore/52630747?from=1&to=704&report=gbwithparts