请帮助分析一下引物吧
丁香园论坛
621
ttattttcag [agtg
8521 gcactgtgga tgctggtgct gacgctggta agaagaaaga tcaaaaggat gataaagtcg
8581 ctgagcaggc ttcaaaggat agggatgtta atgctggaac ttcaggaaca ttctcagttc
8641 cacgaataaa tgctatggcc acaaaacttc aatatccaag gatgagggga gaggtggttg
8701 taaacttgaa tcacctttta ggatacaagc cacagcaaat tgatttgtca aatgctcgag
8761 ccacacatga gcagtttgcc gcgtggcatc aggcagtgat gacagcctat ggagtgaatg
8821 aagagcaaat gaaaatattg ctaaatggat ttatggtgtg gtgcatagaa aatgggactt
8881 ccccaaattt gaacggaact tgggttatga tggatggtga ggatcaagtt tcatacccgc
8941 tgaaaccaat ggttgaaaac gcgcagccaa cactgaggca aattatgaca cacttcagtg
9001 acctggctga agcgtatatt gagatgagga atagggagcg accatacatg cctaggtatg
9061 gtctacagag aaacattaca gacatgagtt tgtcacgcta tgcgttcgac ttctatgagc
9121 taacttcaaa aacacctgtt agagcgaggg aggcgcatat gcaaatgaaa gctgctgcag
9181 tacgaaacag tggaactagg ttatttggtc ttgatggcaa cgtgggtact gcagaggaag
9241 acactgaacg gcacacagcg cacgatgtga accgtaacat gcacacacta ttaggggtcc
9301 gccag] tgatagtttc
[]里是从网上钓出的一个基因的全长,我的两对能否用?
第一对Forward:
ATG TAT TTT CAG AGT GGC ACT G
Inverse:
CTA TCA CTG GCG GAC CCC TAA
第二对:
上游TATGGCCACAAAACTTCAAT
下游 ctatcactggcggaccc
请问这两对引物可以用吗?谢谢!
8521 gcactgtgga tgctggtgct gacgctggta agaagaaaga tcaaaaggat gataaagtcg
8581 ctgagcaggc ttcaaaggat agggatgtta atgctggaac ttcaggaaca ttctcagttc
8641 cacgaataaa tgctatggcc acaaaacttc aatatccaag gatgagggga gaggtggttg
8701 taaacttgaa tcacctttta ggatacaagc cacagcaaat tgatttgtca aatgctcgag
8761 ccacacatga gcagtttgcc gcgtggcatc aggcagtgat gacagcctat ggagtgaatg
8821 aagagcaaat gaaaatattg ctaaatggat ttatggtgtg gtgcatagaa aatgggactt
8881 ccccaaattt gaacggaact tgggttatga tggatggtga ggatcaagtt tcatacccgc
8941 tgaaaccaat ggttgaaaac gcgcagccaa cactgaggca aattatgaca cacttcagtg
9001 acctggctga agcgtatatt gagatgagga atagggagcg accatacatg cctaggtatg
9061 gtctacagag aaacattaca gacatgagtt tgtcacgcta tgcgttcgac ttctatgagc
9121 taacttcaaa aacacctgtt agagcgaggg aggcgcatat gcaaatgaaa gctgctgcag
9181 tacgaaacag tggaactagg ttatttggtc ttgatggcaa cgtgggtact gcagaggaag
9241 acactgaacg gcacacagcg cacgatgtga accgtaacat gcacacacta ttaggggtcc
9301 gccag] tgatagtttc
[]里是从网上钓出的一个基因的全长,我的两对能否用?
第一对Forward:
ATG TAT TTT CAG AGT GGC ACT G
Inverse:
CTA TCA CTG GCG GAC CCC TAA
第二对:
上游TATGGCCACAAAACTTCAAT
下游 ctatcactggcggaccc
请问这两对引物可以用吗?谢谢!