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Microchip Capillary Electrophoresis

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Microchip capillary electrophoresis (MCE) is gaining popularity due to the developments of simple microfabrication methods under nonstringent laboratory conditions. Moreover, the low material and production costs of polymer-based microchips have further stimulated advances in the applications of MCE in various fields, including clinical analysis, drug screening, biomarker identification, and biosensing. In this chapter, a simple and robust protocol for fabrication of microchips for lab-on-chip testing and microchip electrophoresis is described. The microchips are hybrid poly(dimethylsiloxane) (PDMS)/glass microchips, which are produced by a combination of photolithography and micromolding processes. This type of microchip has been used in a wide range of analyses.
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