【共享】巨噬细胞吞噬荧光微球试验-Phagocytosis and Microparticles

最近接到流式细胞仪检测巨噬细胞吞噬荧光微球试验这么个活，既不熟悉流式也不熟悉吞噬细胞，彻底茫然状态。

连该买哪种微球都搞不清，微球好贵又不敢乱试。

苦找了很久在一些微球公司的网页找到一点自认很有用的文件，拿出来共享，希望与我遇到同样麻烦的同仁看了能有帮助。
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Synthetic polymeric particles have been used extensively to study phagocytosis. Some of these are styrene-acrylamide copolymer latices, polyacrolein cellulose, and polyactic / glycolic acid polymers.Polystyrene particles are the most often used due to their availability, uniform size, stability, and non-toxic properties. When a small particle that has a narrow coefficient of variation for size is used, as few as one particle per cell can be used and the number of particles ingested per cell can be determined. Piskin et al. conducted studies of phagocytosis by blood cells and mouse peritoneal macrophages using polystyrene microspheres of various sizes and surface properties. Their results illustrate several important points. The first is that the number of microbeads phagocytosed per cell was greatest for the smallest particle they used (0.9?m). Second, the number of particles phagocytosed declined as particle size increased to 6.0?m. Still, both leukocytes and macrophages were able to internalize one or two particles of 4-6?m. Third, the more hydrophobic the particles, the more readily they were phagocytosed. The presences of positively charged amino groups on the less hydrophobic particles increased their uptake. Conversely, the presence of negatively charged carboxyl groups lowered the number of particles internalized. Fourth, coating particles with bovine serum albumin significantly reduced the number of particles internalized whereas coating with fibronectin dramatically increased the phagocytosis of particles.

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