Isotope-Based Quantitation of Uptake, Release, and Metabolism of Glutamate and Glucose in Cultured Astrocytes
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Protocols are described for measurement in primary cultures of astrocytes of unidirectional fluxes of glutamate (influx and
efflux), glutamate metabolism to glutamine or CO2
, glucose influx, glycolysis, pyruvate dehydrogenation, oxidative metabolism of glucose, pyruvate carboxylation, glycogen
synthesis, and glycogenolysis. References are made to the in vivo situation, and the importance of using metabolically competent
cultures is emphasized.
