Ten-Minute Electrotransformation of Saccharomyces cerevisiae

The introduction of exogenous DNA into prokaryotic and eukaryotic cells is one of the most frequently used procedures in the daily laboratory work of a molecular geneticist. In recent years, methods based on high-voltage electric shocks have been established for various species (reviewed in this book). In the case of Saccharomyces cerevisiae , several procedures have been described that emphasize maximal transformation efficiency (1 –5 ). In this case, many parameters had to be optimized that require accurate, and consequently time-consuming, controls.