Assays for LH, FSH, and Prolactin

In the clinical laboratory the reproductive hormones are probably the most commonly measured hormones after the thyroid hormones. More than 300 laboratories participate in the UK National External Quality Control Scheme. In addition, investigations into reproduction and fertility in humans and animals remains a major area of research. Illustrative methods are described for the three reproductive hormones (leutinizing hormone, follicle-stimulating hormone, prolactin). Radioimmunoassay, immunoradiometric assay, and enzyme assays are described to give a wide choice of assay formats. There are many commercial assays available and illustrative ones are described. Possible interferences are discussed and procedures for investigating their presence and removal are given.