Immunocytochemistry of NMDA Receptors

This chapter covers the major immunocytochemical methods used in our laboratory for NMDA receptor localization. There are three methods: pre-embedding immunoperoxidase for light microscopy (LM), the same for electron microscopy (EM), and postembedding immunogold (1 –5 ). General versions of these techniques, as well as related techniques (immunofluorescence, immunocytochemistry of cultures) have been discussed and compared for immunolabeling of all types of glutamate receptors in Petralia and Wenthold (6 ). Also, for more information on interpretation of immunolabeling data for glutamate receptors, see Petralia and Wenthold (7 ) and Petralia (8 ). Various modifications of these methods have been described briefly (9 –17 ).