丁香实验_LOGO
登录
提问
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

An Outgrowth Culture System of Normal Human Epithelium: An In Vitro Model to Study the Coordination of Cellular Migration and Proliferation

互联网

348
In order to study the growth regulation of normal human epithelium, we have developed an outgrowth culture system ( 1 ). Cinematographic observation has revealed that the cells migrate and proliferate in a coordinated manner. For example, an outgrowth will expand concentrically as a cell sheet, and the cell density will be consistent between different sized outgrowths ( see Fig. 1 ). The increment in the radius of the outgrowth stays constant (about 1.5 mm/d) and is independent of the size of the explant or donor individuals. We can easily obtain 10 8 cells per specimen in 3 wk. Because of its constant growth pattern, the growth history of the cultures can be easily traced, and comparable cultures can be used for further analyses ( see Note 1 ).
Fig. 1  Macroscopic appearance of outgrowth cultured in DMMC medium supplemented with 4% FBS. Concentric circles are the records of outgrowth expansion made every 2 d.

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序