Protocol for the Use of Enzyme-Linked Hybridization Assays for Genital Ulcer Disease

Etiologic agents of genital ulcer disease include herpes simplex 1 and 2, Treponema pallidum pallidum , Haemophilus ducreyi , and Klebsiella granulomatis . The advent of PCR has allowed for more rapid and sensitive detection of microbial pathogens. In this protocol, we describe the simultaneous detection of these five pathogens and an internal control using a single-tube multiplex PCR and colorimetric enzyme-linked amplicon hybridization assay.