Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling
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The dynamic addition of O -GlcNAc to target proteins is now recognized as a major signaling paradigm impacting phosphorylation, protein turnover, gene expression, and other posttranslational modifications influencing epigenetics. Here we describe the production of and methods for assay of the recombinant enzymes of O -GlcNAc cycling: O -linked GlcNAc Transferase (OGT) and O -GlcNAcase (OGA).
![lpxC/lpxC蛋白Recombinant Acinetobacter baumannii UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)重组蛋白UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase (UDP-3-O-acyl-GlcNAc deacetylase)蛋白](https://img1.dxycdn.com/p/s14/2024/0914/416/2705133163069384381.jpg!wh200)
![lpxC/lpxC蛋白/UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase (UDP-3-O-acyl-GlcNAc deacetylase)蛋白/Recombinant Acinetobacter baumannii UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)重组蛋白](https://img1.dxycdn.com/p/s14/2024/0807/931/4874189879496701281.jpg!wh200)
