DNA的诱变和甲基化

 

・         In Vitro Mutagenesis Using Altered Sites (Bowtell Lab) 

• In vitro Mutagenesis with dut ung single stranded DNA (Hahn Lab)

・         Site-directed Mutagenesis using PCR (Lazo Lab)
PCR site-directed methods allow site-specific mutations to be incorporated in virtually any double-stranded plasmid; eliminating the need for M13-based vectors or single-stranded rescue.

・         Site-directed mutagenesis (Dr. Chastain)

・           Site Directed Mutagenesis Using the Ung-Dut System (Bowtell Lab)

・         Oligonucleotide-directed mutagenesis (Kunkel method) (Jeff NEwman)

・         Site-directed Mutagenesis (Promega)
For generation and selection of oligonucleotide-directed mutants

・         Site Directed Mutagenesis Using the Ung-Dut System (PMCI Research)

・         Nested Deletions (Bowtell Lab)
Nested Deletions using exonuclease-III and mung bean nuclease

・         Deletion Mutagenesis (Promega)
For construction of plasmid or M13 subclones containing progressive unidirectional deletions of  inserted DNA, Including methods for DNA preparation, vector digestion, exonuclease deletion, ligation,  transformation...

・         ExoIII/S1 Deletion (Fermentas)
For construction of plasmid or M13 subclones with nested unidirectional deletions. The method is applicable for DNA sequencing, mapping of boundaries of regions involved in genetic control, DNA/protein interaction sites, or protein domains.

・         Exo/S1 Deletion Series (Vesicle Trafficking)

 

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Four Primer Mutagenesis (PDF) (Templenton Lab)
A PCR-based mutagenesis, based on Sarkar and Sommer, but with no ligation required. 

・         Nested Deletions Using Exonuclease-III and Mung Bean Nuclease (PMCI Research)

・         In vitro Mutagenesis Using Altered Sites (PMCI Research)

・         Unidirectional deletions (erase-a-base) (Crawford Lab)

 

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Design of Mutagenic Oligonucleotides (Promega)

Methylation analysis

・         Bisulfite Treatment of DNA (Issa Lab)

・         Methylation-Sensitive PCR (MSP) (Issa Lab)

・         Bisulfite-PCR (for restriction and/or sequencing) (Issa Lab)

・         Southern Blot Analysis (Issa Lab)

・         Methylated CpG Island Amplification (MCA) (Issa Lab)

・         DNA-Methyltransferase Assay (Issa Lab)

・         Bisulfite Modification (TTO)
Modify DNA with bisulfite for methylation analysis