DNA微序列技术

 

・         Protocols for Making Drosophila Arrays (Stanford U.)
Detailed protocol for making arrays including PCR Amplification of cDNAs for Printing,  Polylysine Slide Preparation, "Post-Processing" and Direct Labeling of cDNA probes.
  

・         Preparation of Slides (Brown Lab)
  

・         Preparation of DNA Samples (Brown Lab)
  

・         Post-Processing of Arrays (Brown Lab)
  

・         Preparation of Fluorescent DNA Probe from Yeast mRNA  (Brown Lab)
  

・         Preparation of Fluorescent Probe from Human RNA (Brown Lab)
  

・         Preparation of Fluorescent Probe from E. coli RNA (Brown Lab)
  

・         Preparation of Fluorescent DNA Probe from Genomic DNA (Brown Lab)
  

・         Cyanine Dye HPLC Purification (Brown Lab)
  

・         Modified Eberwine ("antisense") RNA Amplification Protocol   (Brown Lab)
  

・         Hybridization of Arrays (Brown Lab)
  

・         Preparation of Total RNA from Cultured Human Cells (Telechem)
  

・         Preparation of PolyA+ mRNA from Total Human RNA (Telechem)
  

・         Amplification and Purification of cDNAs for Microarray Manufacture (Telechem)
 

・         Microarray Manufacture and Processing (Telechem)
  

・         Generating Control mRNAs by In Vitro Transcription (Telechem)
  

・         Generating Fluorescent cDNA Controls by Linear PCR (Telechem)
  

・         Preparation of Fluorescent Probes from Total Human mRNA (Telechem)
 

・         cDNA Microarray Hybridization and Washing (Telechem)
 

・         Gene Expression Analysis with Microarrays (Telechem)
 

・         Mutation Detection with Oligonucleotide Microarrays (Telechem)
  

・         Comparative Gene Expression Study Using Microarray (Jeremy Buhler) 
   

・         Microarray Hybridization Protocols (Childs / Kucherlapati Lab)