常用的细胞因子引物表
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11761
|
细胞因子
|
引物序列
|
产物(bp)
|
|
IL-1a
|
5’ ATGGCCAAAGTTCGAGACATG
3’ CTACGCCTGGTTTTCCAGTATCTGAAAGTCAGT
|
816
|
|
IL-1b
|
5’ ATGGCAGAAGTACCTAAGCTC
3’ TTAGGAAGACACAAATTGCATGGTGAACTCAGT
|
810
|
|
IL-2
|
5’ ATGTACAGGATGCAACTCCTG
3’ TCAAGTCAGTGTTGAGATGATGCTTTGACAAAA
|
462
|
|
|
5’ TAACCTCAACTCCTGCCACAATGTACA
3’ ATGTTGTTTCAGATCCCTTTAGTTCCAGA
|
386
|
|
IL-3
|
5’ ATGAGCCGCCTGCCCGTCCTG
3’ AAGATCGCGAGGCTCAAGTCGTCTGTTG
|
459
|
|
IL-4
|
5’ ATGGGTCTCACCTCCCAACTG
3’ TCAGCTCGAACACTTTGAATATTTCTCTCTCAT
|
462
|
|
|
5’ TCTCACCTCCCAACTGCTTC
3’ AGAGGTTCCTGTCGAGCCGTTTCA
|
337
|
|
IL-5
|
5’ ATGAGGATGCTTCTGCATTTG
3’ TCAACTTTCTATTATCCACTCGGTGTTCATTAC
|
414
|
|
IL-6
|
5’ ATGAACTCCTTCTCCACAAGC
3’ CTACATTTGCCGAAGAGCCCTCAGGCTGGACTG
|
639
|
|
IL-7
|
5’ ATGTTCCATGTTTCTTTTAGG
3’ AGCTTTTCTTTAGTGCCCATCAAAATTTTATTCCAACA
|
707
|
|
IL-8
|
5’ ATGACTTCCAAGCTGGCCGTG
3’ TTATGAATTCTCAGCCCTCTTCAAAAACTTCTC
|
302
|
|
IL-9
|
5’ CATCTTTTCTGAGGAATG(AG)TATTTC
3’ AAAACCCTGACATCAAACTGATACC
|
110
|
|
IL-10
|
5’ AGGGCACCCAGTCTGAGAACA
3’ CGGCCTTGCTCTTGTTTTCAC
|
351
|
|
IFN-g
|
5’ ATGAAATATACAAGTTATATG
3’ TTACTGGGATGCTCTTCGACCTCGAAACAGCAT
|
501
|
|
|
5’ TTTTCAGCTCTGCATCGTTTTGGGT
3’ CCTTGAAACAGCATCTGACTCCTT
|
456
|
|
IFN-a
|
5’ ATGAGCACTGAAAGCATGATC
3’ TCACAGGGCAATGATCCCAAAGTAGACCTGCCC
|
702
|
|
IFN-b
|
5’ ATGACACCACCTGAACGTCTCTTC
3’ CTACAGAGCGAAGGCTCCAAAGAAGACAGTACT
|
608
|
|
TNF-a
|
5’ ATGAGCACTGAAAGCATGATC
3’ TCACAGGGCAATGATCCCAAAGTAGACCTGCCC
|
702
|
|
|
5’ AGGCGGTGCTTGTTCCTCA
3’ GTTCGAGAAGATGATCTGACTGCC
|
|
|
TNF-b
|
5’ ATGACACCACCTGAACGTCTCTTC
3’ CTACAGAGCGAAGGCTCCAAAGAAGACAGTACT
|
608
|
|
TGF-b1
|
5’ AACATGATCGTGCGCTCTGCAAGTGCAGC
3’ AAGGAATAGTGCAGACAGGCAGGA
|
200
|
|
b-Actin
|
5’ GTGGGGCGCCCCAGGCACCA
3’ CTCCTTAATGTCACGCACGATTTC
|
548
|
|
|
5’ ATTGCCGACAGGATGCAGAA
3’ GCTGATCCACATCTGCTGGA
|
150
|
