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Outer Membrane Protein Purification

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The major outer membrane proteins (OMPs) from Neisseria meningitidis , which are expressed at high levels, are subdivided in five classes based on molecular weight (1 ,2 ) (see Table 1 ).
Table 1  Major Meningococcal Outer-Membrane Proteins

Outer-membrane proteins

Name

Molecular maass

Function/characteristics

Class 1

PorA

44–47 kDa

Por in

Class 2/3

PorB

37–42 kDa

Por in

Class 4

Rmp

 

R eductionm odifiablep rotein, unknown

Class 5

Opa

26–30 kDa

Adhesion,opa city protein

Opc

25 kDa

Invasion, opacity protein

Iron-regulated proteins

Mirp

37 kDa

Iron acquisition (?);m ajori ron-r egulatedp rotein

FrpB

70 kDa

Ferric enterobactin receptor (also FetA)

Adapted from ref. (1 ).
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