Direct Polymerase Chain Reaction Sequencing with Denaturants
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The advance of Tag -based polymerase chain reaction (PCR) technology (1 –3 ) has had a tremendously positive impact on biomedical research. The combination of PCR and sequencing further revolutionized biological research (3 –11 ). There are two general approaches for sequencing the DNA products amplified by PCR from cDNA or genomic DNA templates.
