【分享】新书：＜PCR Technology:Current Innovations＞

PCR Technology: Current Innovations, Second Edition is a fundamental reference for scientists new to PCR technology and a source of up-to-date applications for those familiar with the method. It provides theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens.

The book begins with discussions of the preparation of PCR experiments, followed by examples of analytical PCR divided into qualitative and quantitative applications. The final section explores preparative methods addressing DNA generation for further analysis and in vitro evolution.

Featuring detailed presentations of PCR protocols, this volume contains critical information for practitioners in a wide variety of fields, including forensics, molecular biology research, clinical DNA diagnostics, botany, and paleontology. It provides the essential tools for developing innovative approaches in this leading technology of the genomics revolution.

目录

I. General Considerations

1. Template Isolation and Preparation
2. PCR Optimization
3. Labeling
4. Cloning

II. Analytical Applications

5. Detection of Nucleic Acid Variation
6. Sequencing
7. In Situ Analysis
8. Quantitative Analysis

III. Preparative Applications

9. Amplification of Unknown Sequences
10. In Vitro Evolution