Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons
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The compartmented culture model utilizing rat sympathetic neurons and sensory neurons is gaining wide use in investigations
of neurotrophic factor signaling and axonal transport. Previous chapters have focused on the preparation of compartmented
cultures. Here we focus on analytical approaches that we have used with compartmented cultures that can be used to investigate
apoptosis, intracellular signaling, and other issues. Techniques used for incubating the cultures with radioisotopes and other
agents, harvesting cell extracts from the cultures, using the cultures for histochemistry, and producing cultures from individual
transgenic mouse pups are described.
