Circular Dichroism of Peptides
互联网
213
Circular dichroism measures the difference between the absorbance of left- and right-handed circularly polarized light, and can be used to monitor the secondary structure of peptides (far UV) and the tertiary structure of larger polypeptides (near UV). This technique is especially useful for helix–coil transitions and other aspects of structural alterations. Data from several low-resolution spectroscopic techniques, including CD, can be combined to generate an overall picture of peptide structure as a function of environmental conditions.
