丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Three Decades of Fungal Transformation: Key Concepts and Applications

互联网

423
Filamentous fungi include a large, heterogeneous group of heterotrophic organisms with profound influence in human activities. In spite of their economic and scientific relevance, little information is available at the molecular level about their biology. The development of genetic transformation protocols has contributed greatly to the molecular dissection of fungal behavior. The use of this approach in combination with large-scale genome sequencing projects has now provided the basis for gaining insight into the function of fungal genes. This chapter reviews the technology of the transformation of filamentous fungi. The protocols for gene transference by protoplasting/PEG, LiAc, electroporation, biolistics and A. tumefaciens are described, and possible mechanisms for transformation are discussed. A brief description of previously reported selection systems is also included. The application of transforming protocols concerning the study of gene function and manipulation are discussed in relation to some fungal species.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序