酵母准备

 

Yeast DNA Preparation

• Yeast Genomic Preparation   (Gottschling Lab)
  Rapid method for yeast genomic DNA isolation
    
• Yeast DNA Preparation (rapid glass bead prep) (Hahn Lab)
    
• Yeast Genomic DNA Preparation (high quality DNA) (Hahn Lab)
    
• Yeast quick plasmid DNA Prep (Hahn Lab)

• Yeast "smash-&-grab" DNA Preparation (Fangman/Brewer lab)
     
• Yeast Genomic DNA Preparation for 2-D Gels (Fangman/Brewer lab)
     
• Preparation of Yeast DNA Embedded in Agarose Plugs (Fangman/Brewer lab)
    
• Yeast Genomic DNA Miniprep (Breeden Lab)
    
• Yeast Chromosome DNA Preparation (Breeden Lab)
    
• Yeast DNA Minipreps (Donis Keller Lab)
  Quick method for preparing yeast genomic DNA
    
• High Molecular Weight Yeast Liquid DNA Preparation (Donis Keller Lab)
  For isolating  intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 µg of DNA per prep.
    
• Crude Purification of "PCR-able DNA" Directly from Yeast Cells (GSCWU)
    
• Restriction Digests of High Molecular Weight Yeast DNA (Donis Keller Lab)
  Restriction digests of YACs for mapping using rare cutting enzymes or more conventional restriction endonuclease digestion.
    
• Isolation of Yeast Genomic DNA (D. Amberg)
    
• DNA Isolation from Yeast (Corbett Lab)
    
• Yeast Miniprep (Corbett Lab)

Yeast RNA Preparation

• Yeast RNA Preparation   (Gottschling Lab)
  Give protocol for RNA preparation using phenol/freezer method and glass bead method
    
• Yeast RNA Miniprep (Breeden Lab)

Yeast Protein Preparation

• Yeast Protein Preparation   (Gottschling Lab)
  Rapid method for yeast genomic DNA isolation
     
• Whole Cell Extracts (Dohlman Lab)
  This is a quick method for extracting total proteins from Yeast for SDS-PAGE and Western Blotting .
    
• Quick Total Yeast Protein Isolation for SDS-PAGE Gels (Dohlman Lab)
  A quick method for extracting total proteins from Yeast for SDS-PAGE and Western Blotting (same protocol as above)
     
• Yeast Protein Preparation for SDS PAGE and Western (rapid) (Haha Lab)
    
• Yeast Protein Preparation for SDS PAGE and Western (Haha Lab)
  Yeast whole cell protein extraction for western blot using glass bead method
   

・         Sucrose Density Gradient Fractionation of Yeast Membranes (Dohlman Lab) 
This is an excellent protocol for localization of membrane associated proteins in yeast.

・         Protein Extraction from S. cerevisiae (Howell Lab)
Glass bead method

・         Extracts for Yeast Westerns  (Herskowitz Lab)

・         Yeast Protein Isolation (Amberg Lab)
Yaffe-Schatz method

・         Yeast Nuclear Extract Prep (small scale) (Hahn Lab)

・         Yeast Whole Cell Extract (small scale) (Hahn Lab)