Mutagenesis of C. elegans
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<center> <h1> Mutagenesis of C. elegans</h1> </center>
Materials:
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ENG plates |
0.5% Peptone 0.1% Yeast Extract 51 mM NaCl 2% agar add after autoclaving: 5 ug cholesterol, 1 mM CaCl2 , 1 mM MgSO4 , 1 mM K-phosphate (pH 6) |
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1 M NaOH |
make up 500 mls in plastic container |
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1 M NaOH waste container |
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M9 Buffer |
22 mM KH2 PO4 42 mM Na2 HPO4 85.5 mM NaCl 1 mM MgSO4 |
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EMS (Ethyl Methanesulfonate) |
(Sigma M-0880) |
