Microglial Activation: Measurement of Cytokines by Flow Cytometry
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Cytokine measurement is a prerequisite to understand the inflammatory state of the body. Quantitative analysis of cytokines by Western blotting and ELISA is a daunting task as these are time-consuming and error-prone protocols. With the advent of flow cytometry, the estimation of cytokines using the classical antigen–antibody reaction has become a popular choice with researchers/clinicians. Here, we describe a protocol for multiple cytokine analysis using flow cytometry.