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Three-Dimensional Imaging of Fetal Mouse Kidneys

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244
Three-dimensional imaging is a valuable tool for analyzing kidney growth and development. This technique provides information about spatial relationships between the branching ureteric bud, nephrons, and other structures within the kidney. Availability of user-friendly volume-rendering software now puts this technique within the capability of most laboratories with access to a confocal microscope. This paper describes how to prepare samples and acquire images and three-dimensional volume-rendered images.
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