In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase
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The native T7 DNA polymerase is a fast and highly processive enzyme that can be used for in situ
detection of apoptosis and various types of DNA breaks. The technique is quick and simple, and was shown to label earlier
stages of apoptosis compared to the terminal transferase technique. The in situ
labeling applications of T7 DNA polymerase are presented and summarized from the DNA damage detection standpoint. The detailed
protocols are provided together with the discussion of their advantages and limitations.
