Serum Apolipoproteins

The apolipoproteins associated with serum lipoprotein particles give structural stability as well as regulatory control in lipid metabolism. The development of atherosclerosis is linked to dysfunction in lipid metabolism, and the serum lipoproteins are directly involved, either through the action of their apolipoprotein components or in combination with the lipids sequestered in these particles. Consequently, quantitative features of the apolipoproteins (apos), serum levels, distribution within the lipoprotein population, and the appearance of isoforms, are potential markers for cardiovascular disease risk that are being added to the lipid profile for more effective cardiovascular risk screening (1 ). Currently, apo quantitation is being carried out using immunoassay or gel electrophoresis for separation and staining with image analysis for quantitation (2 ). Capillary electrophoresis has the potential for higher resolution, greater specificity, speed, and automation, coupled with on-line detection for more accurate and precise quantitation.